We analyze mitochondrial dysfunction

We analyze oxidative stress consequences

We analyze cell metabolic imbalances

We analyze functional metabolism in cell cultures

We analyze cell metabolism in tissue extracts

We analyze cell metabolism in various species

From caenorrhabditis elegans, to flies, mice and humans

WELCOME TO INTEGRACELL

Our CRO develops unique cell metabolism exploration services to better understand cell behavior

OUR PORTFOLIO OF TESTS

INTEGRACELL has developed a fee-for-service based platform for cell metabolism exploration on either in vitro or in vivo models 

 

Cell metabolism comprises a series of daedalian biochemical reactions catalysed by different enzymes, each working with an ultimate goal of either synthetizing complex molecules from simple ones or breaking down complex to simpler ones. INTEGRACELL has developed an extensive repertoire of high-throughput suitable quantitative panel of biological assays allowing an important functional metabolism exploration of several central cell metabolisms.

Oxidative Stress

 

Enzymatic activities

Cu/Zn Superoxide dismutase (SOD1)

Mn Superoxide dismutase (SOD2)

Catalase (CAT)

Glutathione peroxidase (GPX)

Glutathione reductase (GR)

Glucose 6P dehydrogenase (G6PD)

Glutathione synthase (GSS)

Glutamylcysteinyl synthase (GCLC)

 

 

  

 

 

Substrates

               Total Glutathione (TG)

Reduced Glutathione (GSH)

Oxidized Glutathione (GSSG)

Malondialdehyde (MDA)

Oxidized LDL (oxLDL)

8-Isoprostane (8-iso-PGF2α)

8-oxo-2′-deoxyguanosine (8-oxo-dG)

 

Global Antioxidant Tests

DPPH

FRAP

 

Mitochondrial function

 

Enzymatic activities

Citrate Synthase (CS)

ETC Complex I (CI)

ETC Complex II (CII)

ETC Complex III (CIII)

ETC Complex IV (CIV)

ATP synthetase Complex V (CV)

 

 

 

 

 

 

 

 Substrates

ATP

 

Central metabolism

 

Enzymatic activities  

Hexokinase (HK)

Fructose 1,6-diP aldolase (ALDO)

Glyceraldehyde 3-P dehydrogenase (GAPDH) 

Pyruvate kinase (PK)

Lactate dehydrogenase (LDH)

Aspartate aminotransferase (ASAT)

Alanine aminotransferase (ALAT)

Gamma-glutamyl peptidase (γGT)

Alkaline phosphatase (ALP)

Glutamate dehydrogenase (GLDH)

Creatine kinase (CK)

 

 Substrates

Lactate

Pyruvate

Malate

Glucose

Acetate

Acetoacetate

Beta-Hydroxybutyrate

Non esterified fatty acids

Triglycerides

Glycerol 

Cholesterol

 

 

NADPH production

 

Enzymatic activities

Glucose 6P dehydrogenase (G6PD)

Malic enzyme (ME)

Isocitrate dehydrogenase (IDH)

 

 Substrates

NADP

NADPH

Energy regulation

 

Insulin

Glucagon

Adiponectin

Leptin

 

 

 

 

 

Miscellaneous cell markers

 

Creatinine

Albumin

Bilirubin

Homocysteine

 

WE EXPLORE TINY cell METABOLISM VARIATIONS

INTEGRACELL platform allows automated quantification of key metabolic parameters with the highest precision

 

As it was not possible at present to measure all cell metabolism parameters for metabolism exploration in parallel but successively, hereby generating an unavoidable degradation of the starting material, we have decided to develop new very precise multiparametric means to be able to assess small metabolic variations in the shortest turnaround time. As it is also possible to measure protein (or hemoglobin or DNA…) content in the same sample, we can not only quantified but also compared the different results between us. Therefore, we can unambiguously define enzyme activities ratios, substrates ratios… that allow a true comparison of cell metabolism pathways function at a precise moment.

 Spider representation of compared OXPHOS & OS metabolisms at 1g/L and 5g/L glucose in culture of two prostate cell lines

(A: PNT2 and B: LNCaP) 1g/L results are given as reference (100%) and 5g/L values are calculated from the reference 

THE FIELDS OUR cell METABOLISM EXPLORATION ADDRESS

INTEGRACELL cell metabolism exploration can help you in different situations

 

Our simple, flexible and versatile metabolism exploration (compatible with many kind of samples such as cells, tissues, organs, culture media, food, serum, plasma, urine… from different species) enables our customers to get a complete picture of the importance their compounds may have on cellular metabolism either in vitro or in vivo. This will help them to make appropriate decisions on go/no-go steps during discovery.

We can help you to better prove your claims

and compare product efficacy

 
 

We can bring you essential data to better select compounds,

then find the best one

 

We can help you to better select compounds,

then find the best one

 

We can help you to better understand

the metabolic part of various pathologies

 
 

We can help you to analyze what can be maximized

and improved to optimize your wellness or performances

 

We can help you to better characterize your cellular models

 

 
 

OUR CLIENTS

OUR PARTNERS